![]() Xotx5b expression is also shown at stage 10.5 (B), where arrows point at Brachet's cleft, and arrowheads show expression in the dorsal neuroectoderm at stage 12 Xotx5b expression is only in anterior presumptive neural ectoderm, but not in the anterior mesendoderm (arrowheads).įig. Xotx5b (A) and Xotx2 (C) expression at stage 10.25 are compared on sections after whole-mount in situ hybridization. At stage 11.5, expression in the dorsal lip is no longer detectable (arrowhead in E, vegetal view) Xotx5b expression in the anterior neuroectoderm is increased at this stage (E, vegetal view) and at stage 12 (F, frontal view).įig. Starting from stage 10.5, expression is also detected dorsally in the anterior neuroectoderm (C, dorsal view D, vegetal view). Expression is detected in the dorsal blastopore lip at stage 10 (A, vegetal view), stage 10+ (B, vegetal view), stage 10.5 (arrowhead in C, dorsal view) and stage 11 (D, vegetal view). Expression of Xotx5b during gastrula stages, as detected by whole-mount in situ hybridization. Genes referenced: ag1 bmp4 crx krt12.4 notch1 nrp1 otx2 sox2 Article Images: show captionsįig. Finally, in einsteck transplantation assays, Xotx5b is able to respecify a tail/trunk organizer to a head organizer. In animal cap assays, Xotx5b and Xotx2 are both able to activate XAG, to strongly suppress the expression of the epidermal marker XK81, and to reciprocally activate each other. Overexpression of Xotx5b has a similar effect to that of Xotx2, producing posterior truncations and inducing ectopic cement gland and neural tissue in whole embryos. After neural tube closure, Xotx5b is expressed in the eye and pineal gland, both involved in photoreception. During neurula stages Xotx5b exhibits a changing and dynamic pattern of expression. Early expression of Xotx5b resembles that of Xotx2, being detected in the organizer region at early gastrula stage, and, shortly after, also in anterior neuroectoderm. We describe the cloning, expression pattern and functional overexpression analysis of Xotx5b, a new member of the Otx gene family in Xenopus laevis. ![]()
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